Figure 3

Disturbing claudin extracellular loop interactions with DFYNP inhibits wound healing. Representative confocal microscopy images of DAPI (nuclei) staining in 16N, T47D, 21MT1, cells, grown on 100 µg/ml collagen type I + 3.5 µg/cm2 Cell-Tak-coated slides (A) at 4 (16N) or 24 (T47D, 21MT1) hours post scratch treatment with control medium (top panel of A) or medium with 400µM DFYNP peptide (bottom panel of A). The mean distance across the gap at 4 (16N, EpH4) or 24 (T47D, 21MT1, MCF-7, OVCAR3) hours post scratch was measured, using SlideBook software, for each cell line for each condition. Mean ± s.e.m., n=3 wells for each treatment (20 measurements taken for each well) *p < 0.05 vs. non-treated.