Figure 5

Claudin-4 knockdown inhibits cell motility. Representative confocal microscopy images (A) of fixed T47D cells and T47D cells with claudin-4 knocked down by shRNA technology [“T47D(-)cld4”] analyzed by immunofluorescence for claudin-4. Western blot analysis (B) confirmed a significant decrease in claudin-4 protein expression in T47D(-)cld4 cells compared to T47D cells. A scratch assay was performed on monolayers of both T47D and T47D(-)cld4 in the absence or presence (+P) of 400 µM DFYNP peptide for 24 hours and the distance across the gap at 24 hours was measured (C). Mean ± s.e.m., n=4 wells for each treatment (20 measurements taken for each well) *p < 0.05 vs. non-peptide treated.