Figure 8

Proliferation does not influence the wound healing response. Representative confocal microscopy images of nuclei (DAPI, red) and Ki67 (yellow) in T47D cells, grown on 100 µg/ml collagen type I + 3.5 µg/cm2 Cell-Tak, 24 hours post scratch in absence or presence of the mimic peptide (A). The size of the gap remaining at 24 hours post scratch treatment was measured for T47D cells plated on Cell-Tak (ctT47D) or collagen (cT47D). After the scratch the media was replaced with either normal media plus (+P) or minus DFYNP peptide in the absence or presence (+m) of mitomycin-C. The inhibition of wound healing with the DFYNP peptide was similar in cells treated with mitomycin-C compared to untreated cells. Mean ± s.e.m., n=3 wells for each treatment (20 measurements taken for each well), *p < 0.05 vs. non-treated, NS=not significant.