Figure 5

O-FISH analyses of viral replicative cDNA during early HIV-1 infection. MT-2 (a, b) and Jurkat (c, d) cells were either mock-infected or infected with HIV-1 and then fixed onto glass slides at 0, 2, 4 or 6 hrs post-infection. Cells were incubated with O-FISH probes to detect either early (strong stop) (a, c) or intermediate (gag) (b, d) HIV-1 cDNA reverse-transcription products (see Figure 2 for relative genome position). Bound probes were detected using the O-FISH method. Quantification of signal events was performed using the spots function in Bitplane Imaris Software. Data shown are mean (+/− SEM) signals per cell, derived from a minimum of 5 images per condition. O-FISH were counted in approx 120–150 cells over 24–36 fields for each of the 4 panels. Paired two-tailed t-tests were performed to determine the significance level of the data sets.