Figure 6

O-FISH analyses of viral replicative cDNA after blocking with AZT reverse transcriptase inhibitor. MT2 cells were infected with HIV-1 in the absence or presence of AZT at 0.1 μg/ml or 10 μg/ml concentration. Cells were fixed onto glass slides at either 20 min or 12 h post-infection and subjected to the O-FISH protocol using probes to detect both strong stop cDNA and gag cDNA. The O-FISH signals were compared to the respective AZT-untreated controls at 12 hrs post-infection. Images were analysed using Imaris software. Data shown are mean (+/− SEM) signals per cell, derived from a minimum of 5 images per condition. O-FISH signals were counted in a total of 158 cells (91 cells over 29 fields for the gag probe and 67 cells over 29 fields for the strong stop probe).