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Figure 7 | BMC Cell Biology

Figure 7

From: Visualising single molecules of HIV-1 and miRNA nucleic acids

Figure 7

O-FISH analyses of RNA conformational rearrangements during early HIV-1 infection. MT2 cells (a) and Jurkat cells (b) were either mock-infected or infected with HIV-1 and then fixed onto glass slides at 0, 2 or 4 hrs post-infection and incubated with a HIV-1 O-FISH probe to detect plus strand U5/PBS RNA(see Figure 2 for relative genome position). Images were analysed using Imaris software. Data shown are mean (+/− SEM) signals per cell, derived from a minimum of 5 images per condition. O-FISH signals were counted in a total of 160 cells (61 cells over 29 fields for the MT2 cells and 91 cells over 20 fields for the Jurkat cells).

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