Skip to main content

Table 1 Effects of high glucose and NOS inhibitor L -NAME on the viability, NO release, eNOS expression and F-actin fluorescence intensity of human umbilical vein endothelial cells incubated in different media (as described in Figure 2 ) for seven days

From: Constant or fluctuating hyperglycemias increases cytomembrane stiffness of human umbilical vein endothelial cells in culture: roles of cytoskeletal rearrangement and nitric oxide synthesis

Group

Cell viability (Optical density)

NO concentration (μmol/L)

eNOS expression (%)

F-actin florescence intensity

Control

0.80±0.093

78.6±4.00 #

73.7±4.40 #

1083±261.7 #

HG

0.64±0.061

47.0±10.56 *

50.5±0.72 *

1538±250.7 *

FG

0.73±0.114

42.4±8.59 *

39.6±0.37 *#

1566±309.3 *

HM

0.50±0.106 *

58.7±13.34 #

57.0±1.38 *#

1057±316.1 #

FM

0.74±0.098

57.1±11.24 #

56.2±1.19 *#

1110±385.5 #

0.1mM

0.65±0.104

50.3±9.24 *

48.5±1.48 *

1501±228.9 *

0.5mM

0.70±0.075

40.9±4.00 *

40.4±0.82 *#

1625±344.8 *

1mM

0.59±0.156

35.4±0.69 *

28.3±0.29 *#

1785±323.4 *#

  1. Data shown in the table are means±SD. *, P<0.05, compared to the control group. #, P <0.05, compared to the constant high glucose group.