Figure 1From: Cell metabolism sets the differences between subpopulations of satellite cells (SCs)Characterization of mitochondrial metabolism differences in both cloned and uncloned satellite cells. (A) Freshly isolated SCs dissociated from single myofibers and seeded on gelatin-coated slides showed at immunofluorescence expression of satellite cell markers Pax7, Myf5 and MyoD (scale bar: 100 μm). (B) Time zero analysis: in 5 out of 5 preliminary experiments, after 24 hours of culture in muscle proliferating medium, SCs presented a different mitochondrial membrane potential ΔΨm (***p < 0.001). (C) Measurement of NaDH level: HPC and LPC demonstrated significant different redox states. These data confirmed that HPC has a glycolytic metabolism compared to low proliferative clones (*p < 0.05). (D) Measurement of CO2 after incubation of D-glucose U-C13 for 4 hours, in SC clones derived from fast and slow-twitch muscle fibers. Treated cells produced a higher amount of CO2 compared to untreated control cells (**p<0.01, ***p < 0.001). Furthermore, HPC showed higher CO2 production in respect to LPC, independently from the muscle type origin (***p < 0.001).Back to article page