Figure 9From: Syndecan-2 is upregulated in colorectal cancer cells through interactions with extracellular matrix produced by stromal fibroblastsEffect of stromal fibroblast ECM on the expression of integrins β1 and α2, and phosphorylation of FAK and Src in HCT-116 cells. (A) HCT-116 cells were cultured for 48 h in the absence of matrix (Ctrl), in the presence of stromal fibroblast ECM (Fibrob. ECM) and then immunostained with anti-integrin-β1, anti-integrin-α2 or anti-phospho-FAK. The staining was analyzed through flow cytometry. (B) The relative staining was determined by densitometric analysis. (C) HCT-116 cells were seeded on stromal fibroblast ECM and cultured for 48 h. Lysate proteins were separated on 10% SDS-PAGE and electro-transferred to PVDF membrane. Membranes were blocked and incubated using anti-FAK, anti-phospho-FAK, anti-Src, anti-phospho-Src and anti-β-actin (loading control). Antibody binding was visualized by chemiluminescence and the relative levels of these proteins were determined by densitometric analysis (D). *p ≤ 0.05 compared to control.Back to article page