Figure 1

Selection of the lentiviral vector harboring siRNA with the highest knockdown efficiency. A, Fluorescence microscopy examination of the infection efficiencies of different lentiviral vectors in JAR cells (magnification 200 ×). a. JAR cells infected with lentiviral particles harboring a non-targeting control siRNA (NC group) in the light microscope; b. JAR cells of NC group in the fluorescence microscope; c. Fusion image of the top two images; d. JAR cells infected with KD3-harboring lentiviral particles (KD group)at a low MOI in the light microscope; e. JAR cells of KD group at a low MOI in the fluorescence microscope; f. Fusion image of the top two images; g. JAR cells infected with KD3-harboring lentiviral particles at a high MOI in the light microscope; h. JAR cells of KD group at a high MOI in the fluorescence microscope. i. Fusion image of the top two images. The fluorescence expression in cells infected with KD1, KD2 and KD4 was similar to that in cells infected with KD3. The infection efficiencies of these lentiviral vectors were about to be above 80%. B, Relative levels of H19 in JAR cells infected with different groups of lentiviral particles at a low MOI. C, Relative levels of H19 in JAR cells infected with different groups of lentiviral particles at a high MOI. Compared to the NC group, H19 expression was significantly downregulated in JAR cells infected with different groups of lentiviral particles, at either a low or high MOI. Beta-actin was used to normalize the PCR data. The highest knockdown efficiency was achieved using KD3-harboring lentiviral particles.