Figure 3

Effect of H19 knockdown on the apoptosis of JAR cells. A. Comparison of the percentages of normal cells and early apoptotic cells between the NC group and the KD group. H19 knockdown significantly increased the percentage of normal cells but decreased the percentage of early apoptotic cells when compared with the NC group; B. Flow cytometric analysis of JAR cells in the NC group and the KD group; C. Comparison of the percentages of normal cells and early apoptotic cells between the two groups with DDP treatment. H19 knockdown also significantly increased the percentage of normal cells but decreased the percentage of early apoptotic cells when compared with the NC-DDP group; D. Flow cytometric analysis of JAR cells in the NC-DDP group and the KD-DDP group. NC-DDP is the negative control vector-harboring cells incubated with DDP; KD-DDP, H19-RNAi-LV-3-infected cells incubated with DDP. ** denotes P < 0.01.