PC cell surface expression in podocytes. (A) Confocal microscopic analysis (×600) for the distribution of PC in HGEC. Cells were fixed and stained with anti-PC antibody and AlexaFluor 488-conjugated anti-mouse IgG as secondary antibody. (B) FACS analysis of PC cell surface expression. Representative experiments from flow cytometric analysis of PC. Thick grey lines represent cells HGEC:5 mM, thin grey lines represent the corresponding isotype control antibody and black lines represent stimuli. (C) Percentage of positive cells was calculated for the histogram section indicated as M1 (*p<0.05 vs. HGEC:5 mM, **p<0.01 vs. HGEC:5 mM, #p<0.05 vs. HGEC:25 mM).