Table 2 Primers used for PCR amplification to construct EGFP-mTOR and mTOR-EGFP (5' – 3' direction; restriction sites underlined)
1_HindIII_FP | GGG AAA TAC GTA AAG CTT AAG ATG GGG CTT GGA ACC GG |
4_SalI_RP | GGG CGG CCG CGT CGA C CC CCA GAA AGG GCA CCA GC |
5_HindIII_FP | GGG AAA TAC GTA AAG CTT TAC TTG GAA CCG GAC CTG C |
8_SalI_RP | GGG GTC GAC TTA CCA GAA AGG GCA CCA GC |