Figure 6From: Chronic glucolipotoxic conditions in pancreatic islets impair insulin secretion due to dysregulated calcium dynamics, glucose responsiveness and mitochondrial activityChronic glucolipotoxic conditions in vitro reduce intracellular insulin content. Rat islets were cultured under normal conditions (control) or with 16.7mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, islets were treated with 2 mM glucose (LG) or 11 mM glucose (HG) for 2 h. Islets were washed, lysed and islet insulin content was measured. Data is expressed as mean±SEM and statistical analysis was performed using the unpaired Student’s t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).Back to article page