Figure 4

PKCθ regulates IRS1 and ERK-mediated differentiation. A) Western blot analysis of AKT s473 and ERK T202/Y204 in scramble and B) PKCθ^shRNAcells treated with insulin and with or without wortmannin and U0126.C) 10x immunoflourescence images of scramble and PKCθ^shRNA cells treated with wortmannin and U0126 (Green; MHC and Blue; nuclei). D) Quantification of the density of MHC+ cells. *; P<0.05 and ***; P<0.0001 compared to wortmannin treated scramble cells. $; P<0.0001 compared to U0126 treated scramble cells. #; P<0.0001 compared to wortmannin treated PKCθ^shRNA cells. E) Number of nuclei per MHC+ cell. ***; P<0.0001 compared to wortmannin treated scramble cells. #; P<0.0001 compared to wortmannin treated PKCθ^shRNA cells. Data ± SEM. n=3 for all experiments.