Figure 3
From: Retinoic acid has different effects on UCP1 expression in mouse and human adipocytes
TTNPB mimics and BMS493 inhibits the effects of ATRA in WT MEFs. Total RNA was harvested at the indicated days (day 8 in panels A and C) and analyzed by RT-qPCR. Relative mRNA expression levels of FABP4, UCP1 and RARβ were determined by normalization to TBP. (A) TTNPB was supplemented to differentiating cells from day −2 to day 8 at the concentrations indicated. (B) TTNPB (1 nM) was acutely supplemented to mature adipocytes (day 8) and harvested after 24 h. (C) Treatment with BMS493 (1 μM) and/or ATRA (1 μM) from day −2 to day 8. (D) Acute supplementation of BMS493 (1 μM) and/or ATRA (1 μM) to mature adipocytes from day 8 and harvested after 24 h. Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells. ▲, p < 0.05 versus ATRA-treated cells.