Activation of PPARδ in WT MEFs does not increase UCP1 expression. Total RNA was harvested at day 8 (panel A) or at the indicated days (panel B) and analyzed by RT-qPCR. Relative mRNA expression levels of FABP4, UCP1 and ADRP were determined by normalization to TBP. (A) Treatment with the PPARδ agonist GW501516 (1 μM) from day −2 to day 8. (B) GW501516 (1 μM) was acutely supplemented to mature adipocytes from day 8 and harvested after 24 h. Data represents mean + SEM (n = 3). *, p < 0.05 versus vehicle-treated cells.