Figure 5

Localization of endogenous E-cadherin in response to PP6c knockdown and casein kinase 1 inhibition. For inducible knock down of PP6c Caco-2 cells were infected with a lentivirus (A) or an adenovirus (B) or subjected to infection with non-coding shRNA virus, as a control. After 4 days the cells were immunostained for indicated proteins and observed with confocal microscopy. Cells were treated with or without 10 μM IC261 for 4 hr. Immunofluorescent images of E-cadherin in control and PP6c KD cells with or without IC261 treatment. (C) Quantification of fluorescence intensity of E-Cadherin in (B) by line scans (10 μm). (D) The full width at half maximum (FWHM) of each line scan was calculated according to description in Methods, and average values of 20 such scans [26] are presented (mean + SE) for each treatment. (E) An immunoblot of PP6c, PP2Ac, E-cadherin and actin in cells with PP6c knockdown compared to control.