In vitro three-dimensional modeling of fallopian tube secretory epithelial cells

Table 2 Summary of immunohistochemical staining of FTSEC spheroids

	FTE02		FTE03		FTE283		FTE284		TISSUE
Marker	2D	3D	2D	3D	2D	3D	2D	3D	FTE03	FTE04
AE1:AE3	++	+/−	+++	+	+++	++++	++++	++	++++	++++
VIMENTIN	+++	++	++++	+++	++++	+++	++++	+++	++++	+++
LAMININ	++++	++++	++++	++++	++++	++++	++++	++++	++	++
MUC-1	++	+/−	+	-	+	+++	++	-	++++	+++
CA125	+	-	+	-	+/−	++	++	-	+++	+++
PAX8	+	-	++	+/−	+++	+++	++	+/−	+++	++
TP53 (Do7)	+++	+/−	+++	+	++	+	+++	+/−	+	+/−
MIB-1 (%)	70%	<5%	70%	<5%	70%	<5%	50%	<5%	<5%	<5%

Immunohistochemistry was performed on five FTSEC cultures grown as 2D monolayers and as 3D spheroids, and compared to two fresh tissue specimens. Staining of pan-cytokeratin (AE1:AE3), mucin-1, CA125, PAX8 and E-Cadherin showed no significant changes in 2D versus 3D cultured cells. Laminin showed high levels of expression in all samples and expression along the basement membrane of fallopian tube epithelial cells in vivo. TP53 and MIB1 expression were high in 2D cultured cells, but low in 3D cultured cells and in vivo.

ISSN: 2661-8850