ADF and cofilin expression levels in rat adenocarcinoma MTLn3 cells. A. Representative 2D Western blot of extracted MTLn3 cell proteins immunolabeled with rabbit polyclonal antibody (1439) that recognizes ADF, phospho-ADF, cofilin, and phospho-cofilin with equal sensitivity. B. Representative Western blots of lysates from control uninfected MTLn3 cells, and cells infected for 72 h with adenovirus for expressing control siRNA, cofilin KD cells (duplicate culture extracts), and ADF KD cells. Blots were probed using the ADF/cofilin pan antibody rabbit 1439 and a monoclonal antibody to GAPDH as a loading control. The experiment was repeated three times and the corresponding quantification is shown in (C). C. Quantification of ADF and cofilin levels in uninfected control, infected (human cofilin siRNA) control, ADF KD or cofilin KD cells. For the uninfected and infected controls the amounts of each shown are as a percent of the total ADF/cofilin. For the ADF KD and cofilin KD samples, the amounts are relative to the control values. *** p < 0.001 versus other treatments.