Skip to main content

Table 2 ADF and cofilin suppression affects MTLn3 cell polarization after EGF stimulation

From: Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells

EGF stimulation

 

Subcategory

Control

ADF KD

Cofilin KD

0 sec

Polarized

Crescent-shaped

68.3 ± 1.6

33.6 ± 1.2***

13.1 ± 0.6***

Kite-shaped

31.7 ± 1.6

66.4 ± 1.2***

86.4 ± 0.6***

Non-polarized

Apolar

91.3 ± 0.3

91.0 ± 0.1

71.5 ± 1.3

Bipolar

8.7 ± 0.3

9.0 ± 0.1

9.7 ± 1.2

Multipolar

0.0

0.0

18.8 ± 1.5

60 sec

Polarized

Crescent-shaped

75.0 ± 1.1

75.2 ± 1.9

49.3 ± 0.7***

Kite-shaped

25.0 ± 1.1

24.8 ± 1.9

50.7 ± 0.7***

Non-polarized

Apolar

91.4 ± 0.3

66.6 ± 0.2***

100.0***

Bipolar

8.6 ± 0.3

34.4 ± 0.2***

0.0

Multipolar

0.0

0.0

0.0

180 sec

Polarized

Crescent-shaped

79.8 ± 0.2

80.6 ± 0.4

48.5 ± 0.5***

Kite-shaped

20.2 ± 0.2

19.4 ± 0.4

51.5 ± 0.5***

Non-polarized

Apolar

91.2 ± 0.3

95.7 ± 0.3***

100.0***

Bipolar

8.8 ± 0.3

4.3 ± 0.3***

0.0

Multipolar

0.0

0.0

0.0

  1. Polarized and non-polarized cells were subcategorized as in Figure 4A and the percentage of cells in each category was scored. Data are expressed as mean ± SEM, n ≥ 250 cells in each experiment, three independent experiments. ***p < 0.001 versus control.