Overexpression of CK1tauinduces long period rhythms. A) Free-running period was analysed in 6 independent transgenic lines overexpressing CK1TAU. Lines were compared against parent line CCA1-LUC in the identical plate position to the transgenic lines. In all cases, a significantly (p < 0.001) long circadian period was observed. B) Examples of traces of overexpression line CK1TAU-OX21 (red) compared to the parent line (black) in free-running conditions. C) Period increase (as in panel A) plotted against overexpression (densitometry of immunoblots, n = 3, as described in the Methods section), showing that in all transgenic lines, overexpression of the tau allele is associated with long period rhythms. D) Subtracted period lengthening of the six CK1tau mutant lines (red bars) compared to the parent line. Combined data of six previously published  independent overexpression lines of the wild-type CK1 allele were plotted for comparison (in blue).