Figure 2

Overexpression of CK1^tauinduces long period rhythms. A) Free-running period was analysed in 6 independent transgenic lines overexpressing CK1^TAU. Lines were compared against parent line CCA1-LUC in the identical plate position to the transgenic lines. In all cases, a significantly (p < 0.001) long circadian period was observed. B) Examples of traces of overexpression line CK1^TAU-OX21 (red) compared to the parent line (black) in free-running conditions. C) Period increase (as in panel A) plotted against overexpression (densitometry of immunoblots, n = 3, as described in the Methods section), showing that in all transgenic lines, overexpression of the tau allele is associated with long period rhythms. D) Subtracted period lengthening of the six CK1^tau mutant lines (red bars) compared to the parent line. Combined data of six previously published [18] independent overexpression lines of the wild-type CK1 allele were plotted for comparison (in blue).