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Table 1 List of predicted CK1 sites present among identified CK1-responsive phospho-sites

From: Functional analysis of the rodent CK1tau mutation in the circadian clock of a marine unicellular alga

Protein description

Site sequence

Gene ID

Closest human relative

E

mi

Accession

Alters Hs clock?

Rhythmic in Mm?

 

Upregulated in both lines:

      

**

***

1

L-asparaginase-like

S(p)LGLLPGPTK

Ot13g02360

L-asparaginase

1e-35

34%

AAH35836.1

Yes (Ï„, A)

Yes (l, a, ba, ag)

2

Armadillo/plakoglobin

AAS(p)AEIASDYVATPGGSR

Ot15g02430

Uridine-Cytidine Kinase 1-like

3e-20

32%

EAW75186.1

-

Yes (ba, a, d)

3

PI-4-phosphate 5-kinase

LRSS(p)VANVTAFATEEPL

Ot02g07550

PI-4-phosphate 5-kinase

2e-06

23%

AAC50916.1

Yes (Ï„, A)

Yes (h, k, a, n)

4

Ankyrin repeats protein

AMQRGSS(p)LLDLQSADGGTPAMSAAAHSYGDVLQYLIEK

Ot08g01070

Ankyrin repeat domain 17

2e-05

38%

AAH43394.1

Yes (Ï„, A)

Yes (bs, l, n, h, s)

5

unnamed protein product

ASTLNDST(p)ADDGNVVR

Ot07g03300

Ankyrin/Armadillo repeats protein

0.014

24%

EAX10893.1

-

-

6

unnamed protein product

EAFGDAS(p)DDDAFSPR

Ot14g01520

Poliovirus receptor-related 4

1.6

32%

BAG61075.1

Yes (Ï„, A)

Yes (n, d)

7

unnamed protein product

ET(p1)KT(p2,3)LAELMS(p3)INMGA*

Ot04g00360

RNF181

1.8

42%

CCQ43543.1

-

-

8

unnamed protein product

GGQEGS(p)PSKSLSSPK

Ot14g02110

No similarity found

N/A

N/A

N/A

N/A

N/A

9

unnamed protein product

ALEDES(p)PVAVVKEK

Ot01g02950

No similarity found

N/A

N/A

N/A

N/A

N/A

10

unnamed protein product

TKDAAEAS(p)DEDVVATR

Ot01g02280

No similarity found

N/A

N/A

N/A

N/A

N/A

 

Upregulated in tau only:

        

11

CDPK

SES(p)FAILTEAAK

Ot10g01030

Death-associated protein kinase 3

2e-37

31%

NP_001339.1

Yes (Ï„, A)

Yes (d, lu, hy)

 

Upregulated in CK1-OX8 only:

        

12

Phox

AIS(p)PAPEER

Ot05g00090

Sorting nexin 1, phox domain

4e-13

22%

NP_003090.2

Yes (Ï„, A)

Yes (d, l)

13

Trehalose-phosphate synthase

PADGST(p)PESPPRR

Ot01g02410

H+ transporting ATPase

5.8

35%

EAW99791.1

Yes (Ï„)

Yes (lu, k, h)

 

Not differentially regulated in either overexpression line:

        

14

putative TAF6 RNA polymerase

GT(p)TPDDDIGDAAAAHAPNVAVAETHV

Ot13g00540

TAF6 RNA polymerase II

4e-65

34%

EAW76588.1

Yes (Ï„, A)

Yes (l, s, p, ag)

15

unnamed protein product

APAGAKPGITLPSNPFAAKPAT(p)KATPAAK

Ot14g01370

Unknown protein

2e-38

40%

AAH53854.1

-

-

16

unnamed protein product

FGIVDGS(p)ASTETPETFVK

Ot08g03890

Spondin 1

0.36

25%

AAH19825.1

Yes (Ï„)

Yes (lu, n, d)

17

unnamed protein product

ALASDS(p)EDDERPR

Ot04g04920

Importin beta

1.1

38%

NP001263382.1

-

-

18

unnamed protein product

TAS(p)PMTSPMASPSPAD

Ot17g01690

2,3-bisphosphoglycerate mutase

9.2

33%

BAD92281.1

Yes (Ï„, A)

Yes (n, sm, p, d)

19

unnamed protein product

SAS(p)YDSLLGAVPASTFPRPIPLAEMVR

Ot15g00280

No similarity found

N/A

N/A

N/A

N/A

N/A

  1. List of CK1 sites based on site prediction that are present in the phospho-preoteome observed in this study and [18]. Parent proteins are listed for each peptide, along with their closest homolog in the human genome. Results are listed based on 1) their presence in significantly differentially regulated sites, and 2) Goodness of homology to closest human ortholog identified by BLAST searches. E values (E) and Maximum Identity (MI) are based on NCBI Blast. *This peptide is identified in three different phosphorylation states (p1, p2, p3), and all three are upregulated upon overexpression of either allele. ** [34], effects on period (t) and/or amplitude (A) upon knock-down in human cells. *** [36–41], rhythmic expression in following mouse cell types: liver (l), aorta (a), brown adipose (ba), adrenal gland (ag), distal colon (d), heart (h), kidney (k), NIH3T3 (n), brain stem (bs), SCN (s), lung (lu), hypothalamus (hy), pituitary (p), skeletal muscle (sm).
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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