mRNA fold change measured by qRT-PCR for osteoblast related genes (continued). A) RUNX2 showed an intense upregulation, increasing almost 400 fold after 30 min, with a drastic descent to levels similar to basal levels after 1 h (p<0.001). B) DLX-5, displayed an increase at 10 min and at 30 min (14.4 fold, p<0.05, 72.9 fold, p<0.001, respectively) reaching a peak at 1 h (135.2 fold, p<0.001), followed by a decrease to basal levels at 2 h. C) OSX showed an increase, from 10 min to 2 h after stimulation (p<0.001). D) SOX9 was upregulated at 30 min (6.4 fold, p<0.05) and 1 h (15.2 fold, p<0.001). Data represent three independent experiments and standard deviation bar is shown for all experimental timepoints studied. Asterisks represents levels of significance for statistical analysis (Two-Way ANOVA, Bonferroni test) between control and treatment groups (*p<0,05 and **p<0,01). ns: non statistically significant.