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Table 3 List of Primers used in qRT-PCR

From: New insights in osteogenic differentiation revealed by mass spectrometric assessment of phosphorylated substrates in murine skin mesenchymal cells

Gene

5′- 3′ nucleotide sequence

mBMP-2 F

TCTTCCGGGAACAGATACAGG

mBMP-2 R

TGGTGTCCAATAGTCTGGTCA

mOsterix F

ATGGCGTCCTCTCTGCTTG

mOsterix R

TGAAAGGTCAGCGTATGGCTT

mRunX-2 F

GACTGTGGTTACCGTCATGGC

mRunX-2 R

ACTTGGTTTTTCATAACAGCGGA

mDlx5 F

CACCACCCGTCTCAGGAATC

mDlx5 R

GCTTTGCCATAAGAAGCAGAGG

mTGFb1 F

CTCCCGTGGCTTCTAGTGC

mTGFb1 R

GCCTTAGTTTGGACAGGATCTG

mSMAD-2 F

ATGTCGTCCATCTTGCCATTC

mSMAD-2 R

AACCGTCCTGTTTTCTTTAGCTT

mTGFbR2 F

CCGCTGCATATCGTCCTGTG

mTGFbR2 R

AGTGGATGGATGGTCCTATTACA

mTwist1 F

GGACAAGCTGAGCAAGATTCA

mTwist1 R

CGGAGAAGGCGTAGCTGAG

mSox9 F

GAGCCGGATCTGAAGAGGGA

mSox9 R

GCTTGACGTGTGGCTTGTTC

mCol4a1 F

CTGGCACAAAAGGGACGAG

mCol4a1 R

ACGTGGCCGAGAATTTCACC

mCol-1 F

GCTCCTCTTAGGGGCCACT

mCol-1 R

ATTGGGGACCCTTAGGCCAT

mCol-2 F

CAGGATGCCCGAAAATTAGGG

mCol-2 R

ACCACGATCACCTCTGGGT

  1. List of primers used to amplify the cDNA in order to investigate changes in mRNA upon BMP2 treatment in qRT-PCR. F: forward; R: reverse.
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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