Experimental strategy and effect of human supplements on MSC proliferation. (A) Experimental strategy and (B) proliferation of MSC affected by the use of different supplements. Cell numbers were determined by the CellTiter-Glo assay on day 3 after seeding 3000 cells/well. Absolute MSC cell counts are shown related to decreasing supplement concentrations of pHPL, tPRP or FBS. Three independent experiments with three replicates were performed (#: p < 0.05; ##: p < 0.01).