Proteomics and western blot verification. (A) Differential 2D-DIGE proteome map of pHPL and tPRP platelet derivatives containing bioactive factors. Differential proteins spots (a total of 47) were detected by utilizing strips from pH 4 to 7 and quantitative Delta 2D imaging software (t-test p ≤ 0.05). Proteins identified by mass spectrometry are specifically shown (no. in rectangle). (B) Complementary Western blot verification of differential proteins in albumin and IgG depleted pHPL and tPRP samples. Different protein concentrations were loaded: α-fibrinogen (2.5 μg protein/lane), α-apoA1 (2.5 μg protein/lane), α-CLIC (5 μg protein/lane), α-annexin A5 (30 μg protein/lane).