Src activity is required for eIF-4 F complex. MDA-MB-231 cells and MDA-MB-435/ β4 cells treated with DMSO or PP2 (A) and infected with either GFP- or Src-shRNA (B) were lysed and then incubated with m7GTP-Sepharose beads. The eluted proteins were analyzed by immunoblotting with antibodies against eIF4G, eIF4E and 4E-BP. The graphs on the right side of each panel represent the mean ratio of 4E-BP1 to eIF4E in triplicate samples (± SD). The statistical analysis was done using Student’s t-test. * P < 0.05, **P < 0.01.