THOC5, a member of the mRNA export complex, contributes to processing of a subset of wingless/integrated (Wnt) target mRNAs and integrity of the gut epithelial barrier

Table 1 RT-PCR primer pair sequences for selected genes

Gene	Acession number	Forward primer	Reverse primer	RT-PCR (spliced)	RT-PCR (unspliced)
Alb (exon2-exon3)	NM_009654.3	CAAGAGTGAGATCGCCCATCG	TTACTTCCTGCACTAATTTGGCA	x
Ascl2(exon1-exon2)	NM_008554.3	AAGCACACCTTGACTGGTACG	AAGTGGACGTTTGCACCTTCA	x
Birc5 (exon3-exon 4)	NM_009689.2	AGCATAGAAAGCACTCCCCTG	CCACTGTCTCCTTCTCTAAGATCC	x
Fn1 (exon41-exon43)	NM_001276408.1	TTCAAGTGTGATCCCCATGAAG	CAGGTCTACGGCAGTTGTCA	x
GAPDH (exon2-exon3)	NM_008084.2	AGGTCGGTGTGAACGGATTTG	TGTAGACCATGTAGTTGAGGTCA	x
GusB (exon1-exon2)	NM_010368.1	CCGACCTCTCGAACAACCG	GCTTCCCGTTCATACCACACC	x
Mmp2 (exon1-exon2)	NM_008610.2	CAAGTTCCCCGGCGATGTC	TTCTGGTCAAGGTCACCTGTC	x
Ptgs2 (exon8-exon9)	NM_011198.3	TTCAACACACTCTATCACTGGC	AGAAGCGTTTGCGGTACTCAT	x
Snai1 (exon2-exon3)	NM_011427.2	CAAGGAGTACCTCAGCCTGG	GGTCAGCAAAAGCACGGTT	x
Sox9 (exon2-exon3)	NM_011448.4	CGGAACAGACTCACATCTCTCC	GCTTGCACGTCGGTTTTGG	x
Sox9 (exon2-intron2)	NC_000077.6	AGTCGGTGAAGAACGGACAAG	GAATGCAAAGTCAGATACTTCTG		x
Beta-actin (exon4-exon5)	NM_007393.3	AACACCCCAGCCATGTACGTAG	GTGTTGGCATAGAGGTCTTTACGG	x
Beta-actin (exon4-Intron4)	AC_000027.1	GCTGTGCTATGTTGCTCTAGACTT	TTTAGATGGAGAAAGGACTAGGC		x
THOC5 (exon1- exon7)	NM_172438.3	TCTGCCTTTTCACCTGGAAG	CTCGGTACTTTTCTGCCAGC	x
Trf (exon1-exon2)	NM_133977.2	ACACACACACCGAGAGGAT	GGTATTCTCGTGCTCTGACAC	x
Wnt11 (exon6-exon7)	NM_009519.2	GGTGGTACACCGGCCTATG	TCACTGCCGTTGGAAGTCTTG	x

Alb (exon2-exon3): albumin (131nt); Ascl2 (exon1-exon2): Achaete scute complex homolog 2 (115nt); ; Birc5 (exon3-exon4): Baculoviral IAP repeat-containing 5 (311nt); Fn1 (exon41-exon43): fibronectin1 (154nt); GAPDH (exon2-exon3): glyceraldehyde-3 phosphate dehydrogenase (123nt); GusB (exon1-exon2): glucouronidase beta (169nt); Mmp2 (exon1-exon2): matrix metallopeptidase 2 (171nt); Ptgs2 (exon8-exon9): prostaglandin-endoperoxidase synthase 2 (271nt); Snai1 (exon2-exon3): snail homolog 1 (179nt); Sox9 (exon2-exon3): SRY-box containing gene 9 (163nt); Sox9 (exon2-intron2) (235nt); beta-actin (exon4-exon5): (509nt); beta-actin (exon4-intron4) (191nt); THOC5 (exon1-exon7): THO complex subunit 5 homolog (643nt); Trf (exon1-exon2): transferrin (125nt); Wnt11 (exon6-exon7): wingless-related MMTV integration site 11 (183nt); (): product size.

ISSN: 2661-8850