Table 2 List of primer pairs selected for RT-qPCR confirmation of BORIS associated transcripts in (A) hNP1 an hNP1 cells differentiated to neurons over 6Â days (6dN), (B) HEK293T cells over-expressing BORIS
From: BORIS/CTCFL is an RNA-binding protein that associates with polysomes
A. | |||
|---|---|---|---|
Gene | Forward Primer | Reverse Primer | Exons |
RNF160 | AAGTTTTGGAAGTATGGAAAACACA | GCTCACTTTGGATGCTTCCTCT | 6-7 |
TTBK2 | ATTGGCTGTGGGAGGAATGA | GTCTACCCAGCCGGAGAGTG | 4-5 |
SKI | CTGGACGACGTGAAGGAGAAA | GGGACTGGGAAGAGGTGTCAT | 1-2 |
PCLO | CACATGCACTGAGTGTCAAACC | CCGCCTAGAGCTCTTTTCATTT | 3-4 |
APC | TGGAAGCAGAGAAAGTACTGGA | GATTCTGAAGTTGAGCGTAATACCA | 4-5 |
BCL2 | CCTGTGGATGACTGAGTACCTGA | TCTTCAGAGACAGCCAGGAGAA | 1-2 |
HIP1 | TGTAAAGGAAAAACACGCCAGA | TGTGGAACACATGGCAGAACTT | 2-3 |
SOX4 | GTTCGGCGTGTGCTTGG | GCAGCGCTTCCGTGTTCT | Â |
APLP2 | GCTCCTGCTTCTGCTGCTG | CCCATTTCCCAGTCTGAATGTT | 1-2 |
CALR | CTCCCGATCCCAGTATCTATGC | TTGTTTCTCTGCTGCCTTTGTT | 7-9 |
POU3F1 | CAACAAGTGGCTGGAGGAGAC | GGCACTTGAGAAAGTGGCTCTC | Â |
B. | |||
Gene | Forward Primer | Reverse Primer | Exons |
CHD8 | CATCGAGTGTTGGATAACTTCTCTG | ATCCATCATCATCAAGGGATCA | 26-28 |
CTBP2 | GAGAGTGATCGTGCGGATAGG | AGAGTCCGCTGTCTCTTCCAC | 5-6 |
CTNNB1 | TGAAAATCCAGCGTGGACAAT | GGTAAGACTGTTGCTGCCAGTG | 2-3 |
FZD5 | CGCTTCTCAGCGGAGTGAC | AGACGGTTAGGGCTCGGATT | 1-2 |
LEF1 | AATGAGAGCGAATGTCGTTGC | TCATAATATTTAGCCTGCTCTTCACG | 7-8 |
LRP6 | TTTATTGGGCAGATGCAAAACTTA | AATAACGTCAAGGCAAAAGGATGT | 3-4 |
TLE1 | TCCCCCTCACATGAGAGTACC | GAAAAGGGACAGGCTGCATCT | 13-14 |
TCF4 | ACCTCTTCCTGTACGCCTCCT | GATCTGGAGAATAGATCGAAGCAA | 11-12 |
TCF3 | TCTCGTCCAGCCCTTCTACC | CGTCCAGGTGGTCTTCTATCTTAC | 13-15 |
WNT5A | CGACATCGAAGGTGGAACTG | CGTTCACCACCCCTGCT | 3-4 |
APC | TGGAAGCAGAGAAAGTACTGGA | GATTCTGAAGTTGAGCGTAATACCA | 4-5 |
TBP | CACGAACCACGGCACTGATT | TTTTCTTGCTGCCAGTCTGGAC | 5-6 |