Figure 3

Differentiation state-distinct contributions of β1 and β4 integrins in the suppression of human IEC anoikis. A. Representative (n ≥ 3) CAD-mediated DNA laddering assays from HIEC (Undifferentiated) and 30PC Caco-2/15 (Differentiated) adhering cell cultures, maintained 24 h serum-free (control) with mouse IgG’s, P4C10 (β1 binding activity-blocking mAb), or 3E1 (β4 binding activity-blocking mAb). L, 100-bp DNA size markers. B. HIEC (Undifferentiated; filled columns) and 30PC Caco-2/15 (Differentiated; open columns) cell cultures were maintained as in ( A ), except that ISEL assays were performed and compared to controls. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 6) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 6) differences between differentiated and undifferentiated IECs are indicated by (#). A-B. Results obtained with -2PC Caco-2/15 cells were highly similar to those shown here for HIEC cells.