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Figure 7 | BMC Cell Biology

Figure 7

From: Suppression of anoikis in human intestinal epithelial cells: differentiation state-selective roles of α2β1, α3β1, α5β1, and α6β4 integrins

Figure 7

Contributions of the α2, α3, and α5 integrin subunits in the engagement of Fak and Src, in human IECs. A. Representative (n ≥ 3) WB analyses of Src and Fak, and verifications of Fak-Src interactions, from adhering HIEC (Undifferentiated) and 30PC Caco-2/15 (Differentiated) cell cultures maintained 24 h serum-free (control) with mouse IgG’s, P1E6 (α2 binding activity-blocking mAb), P1B5 (α3 binding activity-blocking mAb), or P1D6 (α5 binding activity-blocking mAb). Specific antibodies for pY576/577p125Fak, pY418p60Src and pY397p125Fak, as well as for respective total protein forms, were used. B-C. HIEC (Undifferentiated; filled columns) and 30PC Caco-2/15 (Differentiated; open columns) cell cultures were maintained and processed as in ( A ), except that the relative pY576/577 levels of Fak ( B ), as well as the relative activation levels of Src ( C ) and Fak ( D ), were established in comparison to controls. A-D. Results obtained with -2PC Caco-2/15 cells were highly similar to those shown here for HIEC cells. B-D. Statistically significant (0.0001 ≤ P ≤ 0.001; n ≥ 3) differences between treated and control cultures are indicated by (*). Statistically significant (0.0005 ≤ P ≤ 0.005; n ≥ 3) differences between differentiated and undifferentiated IECs are indicated by (#).

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