Figure 8

Effect of level of confluence and culturing time of PKC on pOat mRNA expression. Color-inverted pictures from representative agarose gels of analyzed samples from cell cultures with varying cell density (A) or with varying times in culture (C); summarized densitometric results from cell cultures with varying cell density (B) or with varying times in culture (D); data were normalized with the respective pGAPDH value and are expressed as means with SEM from 3-4 (B) or 4 (D) independent experimentations vs. positive control (pKidney, RNA from porcine renal cortex); level of confluence: 100% represent confluent cultures, values larger than 100% represent postconfluent culture states.