Figure 1
From: ERAD and protein import defects in a sec61 mutant lacking ER-lumenal loop 7
Identification of a sec61 mutant lacking L7. A) Topology model of Sec61p including positions of introduced restriction sites. B) Yeast (SEC61, sec61∆L7, DER1 and ∆der1) were grown as described in Materials & Methods, transferred to nitrocellulose, lysed, and intracellular CPY* detected by immunoblotting. Note that in the strain used for the SEC61 mutagenesis there was higher background accumulation of CPY* compared to the DER1 strain (compare SEC61 and DER1). C) Amino acid sequences of L7 and adjacent regions (grey) in wildtype Sec61p and Sec61∆L7p.