Figure 4

Effect of Stim2 silencing on basal Ca²⁺in HC11 cells. Calcium assays were conducted to assess the basal Ca²⁺ influx in proliferating and differentiated HC11 cells treated with siNT or siStim2. A Basal Ca²⁺ influx is shown as a trace of mean fluorescence relative to baseline in proliferating and differentiated HC11 cells treated with siNT and siStim2. B Rate of basal Ca²⁺ influx, calculated as the slope between 0–5 s after 2 mM Ca²⁺ addition, in differentiated and proliferating HC11 cells with siNT or siStim2. Rate is expressed as a percentage relative to differentiated cells treated with siNT and CPA (n = 4; mean ± SEM, *P < 0.05).