Figure 8

Effect of differentiation in HC11 cells on mRNA expression of Orai1, Stim1, Stim2 and SPCA2. HC11 cells were treated to induce differentiation or kept in maintenance media to keep them proliferating. Total mRNA was isolated 144 h post plating and assessed for Orai1 (A), Stim1 (B), Stim2 (C) and Spca2 (D) levels. Data were normalized to Ppib and Actb mRNA, and are shown as relative fold expression to differentiated HC11 cells. (n = 4; mean ± SD, *P < 0.05, ns denotes no significant difference).