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Figure 4 | BMC Cell Biology

Figure 4

From: Increased protein aggregation in Zucker Diabetic Fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide

Figure 4

Thiol groups and CBS (cystathionine beta synthase) expression in ZDF (zucker fatty diabetic rat) and Lean frontal brain. (A) CBS expression is lower in ZDF brains. (B) Real-time PCR analysis of the expression of CBS relative to β-actin, and (C) Thiol group level in ZDF is lower than Lean. Western blot data showing protein of interest (upper part) and β-actin (lower part). Data are Means ± SEM (n  5 per group), * <0.0001 (difference to control gray bars), magnification 40×; unpaired t-test. Western blot expression is normalized to β-actin; lanes of western blot insets are in the same order as in the X-axis.

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