Thiol groups and CBS (cystathionine beta synthase) expression in ZDF (zucker fatty diabetic rat) and Lean frontal brain. (A) CBS expression is lower in ZDF brains. (B) Real-time PCR analysis of the expression of CBS relative to β-actin, and (C) Thiol group level in ZDF is lower than Lean. Western blot data showing protein of interest (upper part) and β-actin (lower part). Data are Means ± SEM (n ≥ 5 per group), * <0.0001 (difference to control gray bars), magnification 40×; unpaired t-test. Western blot expression is normalized to β-actin; lanes of western blot insets are in the same order as in the X-axis.