Treatment with NaHS counteracts protein aggregates in cultured brain slices from ZDF (zucker diabetic fatty rat). (A) ZDF brain slices retain protein aggregates in culture. Protein aggregates are diminished through NaHS treatment. NAC diminishes protein aggregates in ZDF. (B) The doubling of the number of aggregates in ZDF brain slices is lowered to Lean levels by NaHS treatment. NAC lowers protein aggregates in ZDF brain but at a much slower rate. (C) NaHS lowers the higher protein synthesis levels in cultured ZDF brains indicated by higher protein/DNA ratio. (D) 65 kDa and 55 kDa tau expression is three times higher in ZDF rats compared to Lean and NaHS treatment normalizes tau levels. Also, hyperphosphorylated tau (AT8) is increased in ZDF brains and normalized by NaHS. (E) Fibronectin expression is doubled in ZDF rats compared to Lean and is normalized by NaHS treatment. (F) Real-time PCR analysis of the expression of total tau relative to β-actin. NaHS lowers total tau expression in ZDF to lower than Lean levels (G) Real-time PCR analysis of the expression of fibronectin relative to β-actin. NaHS lowers fibronectin expression in ZDF to lower than Lean levels. Western blot data showing protein of interest (upper part) and β-actin (lower part) from the same samples. Data are means ± SEM (n ≥ 5 per group) *, #, + <0.05 = statistically different, * different from control gray bars in each group, # different from ZDF group, + different from lean, ZDF and ZDF + H2S; One way ANOVA. Western blot expression is normalized to β-actin; lanes of western blot insets are in the same order as in the X-axis.