Figure 2From: A novel early precursor cell population from rat bone marrow promotes angiogenesis in vitroThe heterogenous cell population can be sorted by the ability of cells of the endothelial lineage to take up ac-LDL 488 via specialized scavenger receptors. (a) Cells were incubated with 2.5 μg/ml ac-LDL-Alexa Fluor® 488 for 4 h at 37°C and 5% CO2. Cells were subsequently washed twice with PBS and successful uptake was visualized by fluorescence microscopy (left: brightfield, right: fluorescence). The scale bar depicts 100 μm. (b) By using cell sorting technology, cells that have taken up the acetylated LDL-AF488 conjugate were successfully separated from the remaining population with a purity of 92%. Cell distributions are depicted in the plots.Back to article page