Both N- and C- terminal halves of RasG are required for optimal folate chemotaxis of axenically grown cells. (A) Expression of RasG/RasD protein chimeras detected by binding of RasG and RasD antibodies specific to the C-terminus. (B) Chemotaxis index protein chimeras and controls [n = 40]. Error bars indicate the 95% confidence interval of the mean. Statistical significance: ns = P > 0.05; *** = P < 0.001; **** = P < 0.0001. Light grey circles show average chemotaxis index of each cell. All strains were grown in axenic conditions.