1) | DMEM + 10% FCS + PS |
2) | DMEM/HAMF12 + Dexamethasone + PS |
3) | DMEM/HAMF12 + Epidermal growth factor + Insulin + PS + Selenium + Transferrin |
4) | AECM + Bovine pituitary extract + Epidermal growth factor + Epinephrine + Hydrocortisone + Insulin + PS + Retinoic acid + Transferrin holo |
5) | DMEM/HAMF12 + Dexamethasone + 5% FCS + PS + Retinoic acid |