Analysis of human breast cancer and background tissues. (A) Q-PCR revealed a significant increase in FAP-α expression with poor patient outcome. (B) Expression was also increased in ER positive and ERβ positive patients. (C) Kaplan-Meier survival curves showing overall survival analysis of patients expressing FAP-α. (D) Kaplan-Meier survival curves showing disease-free survival analysis of patients with breast cancer. (E) Immunohistochemical staining of FAP-α in background breast tissues (left) and tumour/associated tissues (right).