Characterization of KIND1 cells differentiated into pancreatic lineage. (A) Expression of pluripotency associated gene transcripts (OCT4, NANOG, SOX2) during differentiation of KIND1 cells from Day 4 – Day 16. (B) Expression of E CADHERIN and N CADHERIN during differentiation in undifferentiated and Day 4 – Day 16. (C) Expression of definitive endoderm specific gene transcripts (SOX17, FOXA2, CXCR4, CERBERUS, EOMESODERMIN) in undifferentiated and Day 4 – Day 16. (D) Expression of primitive gut tube marker HNF4A in undifferentiated and Day 4 – Day 16. The expression of all gene transcripts is relative to undifferentiated KIND1 cells (set as 1). Error bars represent ± SEM, statistical significance represented as *(p <0.05).