Figure 3
Characterization of KIND1 cells differentiated into pancreatic lineage by directed differentiation approach . (A) Expression of pancreas specific gene transcripts (PDX1. SOX9, NKX6.1) in undifferentiated and Day 4 – Day 16. (B) Expression of representative gene transcripts of ectoderm (MAP2) and mesoderm (HAND1, MESP1) in undifferentiated and Day 4 – Day 16. The expression is relative to undifferentiated KIND1 cells (set as 1). Error bars represent ± SEM, statistical significance represented as *(p < 0.05) and **(p < 0.02). (C) Western Blot analysis for SOX17 protein in cell lysates from undifferentiated (UD), Day 4 (D4), Day 8 (D8), and Day 12 (D12) samples, with β ACTIN as housekeeping protein. (D) Western Blot analysis for PDX1 and SOX9 protein in cell lysates from undifferentiated (UD), Definitive Endoderm Day 4 (D4), Primitive Gut Tube Day 8 (D8), and pancreatic progenitors Day 12 (D12) - Day 16 (D16) samples, with β ACTIN as housekeeping protein.