Figure 1From: Differential effects of PDCD4 depletion on protein synthesis in myoblast and myotubesIn L6 myotubes, PDCD4 abundance, but not phosphorylation, is sensitive to medium nutrient composition and requires mTORC1 function. L6 Myotubes were incubated in differentiation (Ctl) or starvation medium (serum-free, amino acid-free RPMI medium) for 12Ā h. They were then harvested (Stv) or refed in the differentiation medium supplemented with DMSO (ā) or 50Ā nM rapamycin (+) for 1 or 3Ā h. (A) Phosphorylated (ph) and total PDCD4 (Ser67), ph-S6K1 (Thr389) and Ī³-tubulin were analyzed by immunoblotting. (B and C) phosphorylated and total PDCD4 signals were expressed relative to Ī³ tubulin. Means Ā± SE of 5ā6 independent experiments; bars with different letters differ (Pā<ā0.05).Back to article page