Figure 6

Morphological changes in HUVEC after exposure to neutrophil MPs and H ₂ O ₂ . HUVEC incubated for 2 min (A) and 16 min (B) with HBSS. Cells were observed with 2 min (C) and 16 min (D) after the addition of H₂O₂. Note the increased blebbing cells observed with 2 min, (E) and 16 min (F) after the addition of 5 μg/mL MPs and 20 μM H₂O₂ (inserts) indicating cell damage. Cells treated with 1 mM taurine showed a lower number of morphological changes with 2 min (G) and 16 min (H). Magnification: 100×. The figures show a representative experiment among four performed.