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Figure 3 | BMC Cell Biology

Figure 3

From: Activation of GPR40 attenuates chronic inflammation induced impact on pancreatic β-cells health and function

Figure 3

Chronic GPR40 activation reverses inflammation mediated increase in oxidative and ER stress. (A) NIT1 cells were treated for 72 h as indicated and ROS levels were measured by DCF fluorescence. ER stress was measured by expression of CHOP (DDIT3/GADD153) gene in rat islets cultured under inflammation with or without GPR40 activator (B). (n = 4, **P < 0.01, ***P < 0.001). (C) NIT1 cells were treated as indicated and total cell lysates were used for western blotting against BiP (GRP78/HSPA5) and p-eIF2a to study ER stress. β-actin and total eIF2a were used as loading control for normalization.

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