Figure 3
From: Recent advances in live cell imaging of hepatoma cells
Oil red O staining for imaging and quantification of lipid droplets in hepatocytes. (A) Oil red O staining of lipid droplets in hepatic cells treated with fatty acid, amiodarone and/or TNF-α. (B) Spectrophotometric quantification of Oil red O stain to determine intracellular lipid load in hepatocytes treated with fatty acids and/or amiodarone. (C) MTT assay to determine cytotoxic effect of fatty acids, and/or amiodarone on cultured hepatocytes.