Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Table 1 Brief summary of various fluorescent proteins developed and their technical details

From: Recent advances in live cell imaging of hepatoma cells

Fluorescent protein Mutations Ex. λ (nm) Em. λ (nm) Photostability Brightness (% of EGFP) Reference
Green Fluorescent protein
wtGFP   395/495 509   48  
EGFP F64L, S65T 488 509 ++++ 100 [19]
Sapphire S72A, Y145F, T203I 399 511 ++   [11]
T-Sapphire Q69M, C70V, V163A, S175G 399 511 ++ 78 [20]
Emerald F64L, S65T, S72A, N149K, M153T, I167T 487 509 +++ 116 [21]
Superfolder S30R, Y39N, F64L, (S65T/G65T), F99S, N105T, Y145F, M153T, V163A, I171V, A206V 485 510 +++ 160 [22]
Blue Fluorescent protein
BFP Y66H, Y145F 360 442 +   
EBFP BFP + F64L, S65T 380 440 + 27 [16]
EBFP 1.2 EBFP + S30R, Y36N, T65S, S72A, N105T, 117IV, N198S, A206V 379 446 ++ 53 [23]
EBFP1.5 EBFP1.2 + F145H, H148N, M153A 381 449 ++ 68 [23]
EBFP2 EBFP1.2 + I128V, V150, D155V, V224R 383 448 +++ 53 [23]
Azurite EBFP + T65S, V150I, V224R 383 447 ++ 43 [24]
Sirius F46L, T65Q, W66F, Q69L, Y145G, H148S, and T203V, F223S 355 424 +++ 12 [25]
Cyan fluorescent protein
ECFP Y66W, F64L, S65T 433/445 475/503 ++ 39 [26]
Cerulean S72A, Y145A, H148D 433 475 +++ 79 [27]
SuperCFP   433 474 ++ 45  
Yellow fluorescent protein
EYFP T203Y 513 527 ++ 151 [28]
Citrine S65G, V68L,S72A, T203Y, Q69M 516 529 ++ 174 [29]
Venus S65G, V68L,S72A, T203Y, F46L, M153T, V163A, S175G 515 528 ++ 156 [30]
Topaz S65G, S72A, T203Y 514 527 ++ 169 [21]