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Figure 3 | BMC Cell Biology

Figure 3

From: Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion

Figure 3

Rols7 is transcribed in TVM and circular visceral muscles. (A) Scheme of the rolling pebbles promoter region. rols7: yellow, 3 kb upstream region required for maximum expression in the somatic mesoderm; green, intron with control elements for transcription in the visceral mesoderm and somatic muscles; blue, exons 1 and 2 of rols7. rols6: pink, approximately 1.2 kb upstream region essential for expression in the endoderm and Malpighian tubules; orange, exons 1 and 2. (B) Expression of the roIsIn1-lacZ reporter construct, which contains the regulatory region between exon 1 and 2 of rols7 (green in A). (B’) Magnification of boxed area in (B); β-Gal (green fluorescence) in longitudinal FCs (arrowheads) along the TVM, marked by anti-FasIII (red fluorescence). (C) β-Gal-positive stretching circular muscles (arrow). (D) In situ hybridization of bHLH54F-lacZ embryos using a rols7 probe (green fluorescence). Longitudinal visceral FCs were stained with anti-β-Gal (red fluorescence). (D’ and D” ) Magnification of C: arrowheads, rols7 mRNA in β-Gal-negative circular visceral FCs; arrows, rols7 mRNA in β-Gal-positive longitudinal FCs. Scale bars: 20 μm.

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